Activiteit

Background The pathologic process of ARVC (arrhythmogenic right ventricular cardiomyopathy) typically originates in the epicardium or subepicardial layers with progression toward endocardium. However, in the most recent ARVC international task force consensus statement, epicardial ventricular tachycardia (VT) ablation is recommended as a Class I indication only in patients with at least one failed endocardial VT ablation attempt. Objective The aim of this meta-analysis is to assess the outcomes of ARVC patients undergoing combined endo-epicardial VT ablation, as compared to endocardial ablation alone. Methods A systematic review of PubMed, Embase, and Cochrane was performed for studies reporting clinical outcomes of endo-epicardial VT ablation vs endocardial-only VT ablation in patients with ARVC. Fixed-Effect model was used if I2 less then 25 and the Random-Effects Model was used if I2 ≥ 25%. Results Nine studies consisting of 452 patients were included (mean age 42.3 ± 5.7 years; 70% male). After a mean follow-up of 48.1 ± 21.5 months, endo-epicardial ablation was associated with 42% relative risk reduction in VA recurrence as opposed to endocardial ablation alone (risk ratio [RR], 0.58; 95% confidence interval [CI], 0.45-0.75; P less then .0001). No significant differences were noted between endo-epicardial and endocardial VT ablation groups in terms of all-cause mortality (RR, 1.19; 95% CI, 0.03-47.08; P = .93) and acute procedural complications (RR, 5.39; 95% CI, 0.60-48.74; P = .13). Conclusions Our findings suggest that in patients with ARVC, endo-epicardial VT ablation is associated with a significant reduction in VA recurrence as opposed to endocardial ablation alone, without a significant difference in all-cause mortality or acute procedural complications.New findings What is the central question of this study? Does short-term high intensity interval training alter microbiome composition, and does this associate with exercise-induced improvements in cardiorespiratory fitness and insulin sensitivity? What is the main finding and its importance? Despite high intensity interval training increasing insulin sensitivity and cardiovascular fitness it did not alter microbiome composition. selleck This suggests microbiome composition changes that occur with prolonged exercise training may respond to changes in metabolic health rather than drive exercise training induced adaptations. Abstract Regular exercise reduces the risk of metabolic diseases, and gut microbiome composition has been associated with metabolic function. We investigated whether short-term high-intensity interval training (HIIT) alters bacterial community diversity and composition, and whether there were associations with markers of insulin sensitivity or aerobic fitness. Cardiorespiratory fitness (VO2 peak ) 5) were observed between the bacterial species Coprococcus_3, Blautia, Lachnospiraceae_ge and Dorea abundance and insulin sensitivity markers in the overweight group. Our results suggest short-term HIIT does not greatly impact the overall composition of the gut microbiome, however certain microbiome genera associate with insulin sensitivity markers that were improved by HIIT in overweight participants. This article is protected by copyright. All rights reserved.The purpose of this study was to investigate the effect of diet supplementations on biochemical, hematological, and redox metabolism parameters in streptozotocin-induced diabetic rats. Healthy male Wistar rats and streptozotocin-induced diabetic rats were provided diets supplemented with 20% of Pinus sp. sawdust or Pycnoporus sanguineus mycelium for 4 weeks. Diabetic rats treated with both Pinus sp. sawdust- and P. sanguineus mycelium-supplemented diets presented a significant decrease in non-HDL cholesterol of 38.43% and 33.53% and triglycerides of 70.03% and 69.81%, respectively, compared to diabetic control. As far as we know, this is the first report of a significant decrease in serum lipids attributed to these supplementations. Even though with the alterations in hematological and redox metabolism parameters related to these diet treatments, our data suggest that Pinus sp. sawdust and Pycnoporus sanguineus mycelium could be a useful a diet supplement to control diabetic dyslipidemia in animals. PRACTICAL APPLICATIONS Pinus sp. sawdust is a residue from the wood industry that can be reused as a substrate to cultivate Pycnoporus sanguineus mycelium. Both species have specific and rich natural compounds. The results of the present study surprisingly showed that diets supplemented with the isolated substrate (Pinus sp. sawdust) and Pycnoporus sanguineus mycelium significantly decrease non-HDL cholesterol and triglycerides in induced diabetic animal model. These new natural approaches can be interesting to develop a nutraceutical to treat dyslipidemia.Background Probiotics regulate host immune balance, which may reduce immune-related diseases. The effects and mechanisms of Lactobacillus rhamnosus 2016SWU.05.0601 (Lr-0601) on immune response in ovalbumin (OVA)-sensitized mice were explored. Results Lr-0601 reduced serum immunoglobulin E (IgE) and OVA-IgE and attenuated the alteration in lung pathology in OVA-sensitized mice. Lr-0601 blocked OVA-induced up-regulation in serum T helper (Th) 2 and Th17 cytokines but increased the serum levels of Th1 and regulatory T (Treg) cytokines in OVA-sensitized mice. OVA also markedly reduced the protein levels of spleen T-box transcription factor (T-bet) and forkhead/winged helix transcription factor p3 (Foxp3), leading to the reduced mRNA expression of interferon-γ (IFN-γ) and interleukin (IL)-10. By contrast, OVA markedly increased the protein expression of spleen GATA-binding protein 3 (GATA3) and retinoid-related orphan receptor γt (RORγt) as well as the mRNA expression of spleen IL-4 and IL-17. These changes induced by OVA were reversed by Lr-0601. Moreover, Lr-0601 helped alleviate OVA-induced intestinal microbiota dysbiosis. Correlation was found between specific genera and immune-associated cytokines. Conclusion The combined results indicate that Lr-0601 modulated the balance of Th1/Th2 and Treg/Th17 in OVA-sensitized mice, which was associated with the regulation of immune-related transcription factors and gut microbiota. Lr-0601 can potentially be used as a probiotic to prevent immune-related diseases. This article is protected by copyright. All rights reserved.