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Background The pathogenesis of adolescent idiopathic scoliosis (AIS), including the role of brain and spinal inhibitory circuits, is still poorly elucidated. The aim of this study was to identify which central inhibitory mechanisms are involved in the pathogenesis of AIS.Design A prospective neurophysiological study, using a battery of neurophysiological tests, such as cutaneous (CuSP) and cortical (CoSP) silent periods, motor evoked potentials (MEP) and paired-pulse transcranial magnetic stimulation (ppTMS).Settings Neurophysiological laboratory.Participants Sixteen patients with AIS (14 females, median age 14.4) and healthy controls.Outcome measures MEPs were obtained after transcranial magnetic stimulation (TMS) and recorded from the abductor pollicis muscle (APB). ppTMS was obtained at interval ratios (ISI) of 1, 2, 3, 6, 10, 15 and 20 ms. The cortical silent period (CoSP) was recorded from the APB. The cutaneous silent period (CuSP) was measured after painful stimuli delivered to the thumb while the subjects maintained voluntary contraction of the intrinsic hand muscles. The data were analyzed and compared with those from healthy subjects.Results The CoSP duration was significantly prolonged in AIS patients. A significantly higher amplitude of ppTMS for ISI was found in all AIS patients, without remarkable left-right side differences. No significant difference in MEP latency or amplitude nor in the CuSP duration was obtained.Conclusion Our observation demonstrates evidence of central nervous system involvement in adolescent idiopathic scoliosis (AIS). Lower intracortical inhibition, higher motor cortex excitability, and preserved spinal inhibitory circuits are the main findings of this study. A possible explanation of these changes could be attributed to impaired sensorimotor integration predominantly at the cortical level.Stabilized leachates from sanitary landfills generally display high levels of recalcitrant organic matter, sometimes requiring a combination of biological and physicochemical treatment processes. This study evaluated the post-treatment by Upflow Gravel Filtration (UGF) followed by Granular Activated Carbon Adsorption (GACA) on a pilot scale of two different landfill leachates previously treated by biological processes. The system design was proven technically feasible for a continuous flow post-treatment in relation to recalcitrant organic matter removal efficiency. The UGF experiments presented 83.9 and 82.0% COD removals for leachates A and B, respectively, with residual values ⁣⁣of 107 and 194 mg L-1. The UGF-GACA experiments, in turn, produced effluents with residual COD values ⁣⁣of 67 and 94.6% efficiencies.The problem of classifying individual finger movements of one hand is focused in this article. The input electromyography signal is processed and eight time-domain features are extracted for classifying hand gestures. The classified finger movements are thumb, middle, index, little, ring, hand close, thumb index, thumb ring, thumb little and thumb middle and the hand grasps are palmar class, spherical class, hook class, cylindrical class, tip class and lateral class. Four state-of-the-art classifiers namely feed forward artificial neural network, cascaded feed forward artificial neural network, deep learning neural network and support vector machine are selected for this work to classify the finger movements and hand grasps using the extracted time-domain features. The experimental results show that the artificial neural network classifier is stabilized at 6 epochs for finger movement dataset and at 4 epochs for hand grasps dataset with low mean square error. Vorinostat However, the support vector machine classifier attains the maximum accuracy of 97.3077% for finger movement dataset and 98.875% for hand grasp dataset which is significantly greater than feed forward artificial neural network, cascaded feed forward artificial neural network and deep learning neural network classifiers.One of the major problems of modern cities is waste management. Cooperation and active public participation are key elements in the effective implementation of waste management programs. The purpose of this work is to investigate the views and attitudes of Athens residents towards recycling, composting and the operation of green centers, which are the key elements of solid waste management as it follows from the recently updated National Waste Management Plan. A structured questionnaire was designed and addressed to the residents of Athens. According to the main findings, half of the respondents are involved in recycling, and the majority of the respondents are willing to participate in future composting programs and have a positive opinion about the operation of green centers. The main obstacle for their further participation is their hesitation regarding the proper operation of the programs (recycling, composting, green centers). There is a need to build up the trust between citizens and the state. Active public involvement is required to successfully implement municipal solid waste strategies during all the phases (planning, siting and operation) in order to ensure public acceptance. In addition, targeted information and awareness campaigns can also contribute to this aim.Human α-thrombin (thrombin) is a multifunctional enzyme that plays a pivotal role in the coagulation pathway. Thrombin activity can be effectively modulated by G-quadruplex-based oligonucleotide aptamers that specifically interact with the two positively charged regions (exosites I and II) on the protein surface. Although insightful atomic-level snapshots of the recognition between thrombin and aptamers have been recently achieved through crystallographic analyses, some dynamic aspects of this interaction have not been fully characterized. We here report molecular dynamics simulations of thrombin in different association states ligand-free and binary/ternary complexes with the aptamers TBA (at exosite I) and HD22_27mer (at exosite II). The simulations carried out on the binary and ternary complexes formed by thrombin with these aptamers provide a dynamic view of the interactions that stabilize them in a crystal-free environment. Interestingly, the analysis of the dynamics of the exosites in different thrombin binding states clearly indicates that the HD22_27mer binding at the exosite II favours conformations of exosite I that are prone to the TBA binding.