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The virulent clade I strains encoded two clusters of type IV secretion systems, while clade II and III strains encoded only one cluster. Clade I and II strains encoded one more VgrG/PAAR spike protein than clade III. Thus, we predicted that the presence of additional virulence-related genes may have enabled the unique clade I strain to become predominant source in the USA outbreak.Passion fruit (Passiflora edulis Sims) is a widely cultivated dicotyledonous perennial plant with woody vines (Asande et al. 2020). In November 2020, leaf blight was observed on leaves of P. edulis (cultivar ‘Panama Red’) newly planted in Wangyou, Huishui county, Guizhou province, China (25°82’57” N, 106°50’49” E). The leaf blight occurred on both young and old leaves, starting from the margins, and then extended to the entire leaves. The color of the affected tissue was brown with a yellow hallo in the early period, and then gradually turned to grey. The disease incidence was 60%-70% on a 0.08-ha field. Following isolation of the potential pathogen from 12 diseased leaves, nine isolates were obtained. The colonies were white with a regular round shape at the early stage and became black with fluffy hyphae after eight days on potato dextrose agar (PDA) medium, incubated at 25°C in the dark for 10 days. The single cell conidia were solitary, spherical or slightly ellipsoidal, black, shiny, smooth, aseptate, sps (cultivar ‘Panama Red’). The same number of leaves from control group plants was only treated with 0.05% Tween buffer. All plants were incubated at 26°C ± 2°C under a 16 h/8 h photoperiod and 70%-75% relative humidity (RH) after inoculation. After 14 days, symptomatic blight appeared on all inoculated leaves. In contrast, no symptoms appeared on leaves in the control group. The disease assays were repeated three times. Pure cultures were re-isolated from diseased leaves and confirmed to be N. sphaerica based on the morphological and molecular methods mentioned above (ITS region, the TUB, and the EF1-α sequences). To our knowledge, this study is the first report of N. sphaerica as a pathogen on P. edulis causing leaf blight. The identification of the pathogen could provide relevant background for its future management.Diabetic foot ulcers (DFUs) remain a common debilitating and costly complication of diabetes mellitus. Indeed, despite all efforts and emerging technologies, many DFUs are difficult to heal and frequently recur. Thus, novel therapeutic approaches are urgently needed. Specific targeting of different molecular and cellular pathways implicated in wound healing emerges as an attractive therapeutic modality to improve outcomes. One of the novel pathways that carry this potential is the wingless-type mouse mammary tumor virus integration site family/beta-catenin signaling pathway (WβcSP). It plays an important role in different stages of wound healing, including inflammation, proliferation, and remodeling. Potential therapeutic implications of WβcSP activation include producing agonists and/or blocking its endogenous inhibitors. Thus, we should perhaps start exploring potential ways of its therapeutic implication to improve DFU healing.Background Finding new approaches to increase health and well-being among nursing home (NH) residents is highly warranted. From a holistic perspective, several Norwegian municipalities have implemented the certification scheme framed “Joy-of-Life Nursing Home” Aims In a holistic perspective on NH care, this study investigated if NH residents despite potential symptom severity experience joy-of-life (JoL). Therefore, we examined the frequency of common symptoms and the association between common symptoms and JoL in cognitively intact NH residents. Methods A cross-sectional design was employed. Using the QLQ-C15-PAL quality-of-life questionnaire, hospital anxiety and depression scale, and JoL scale, a total of 188 cognitively intact NH residents participated. Results Symptom severity was high; 54% reported fatigue, 52% reported constipation, 45% reported pain, 43% reported dyspnea, 32% reported insomnia, 22% reported appetite loss, and 20% reported nausea, while 20% reported anxiety and 23% reported depressive symptoms. Nevertheless, 59% of the NH residents reported high JoL, which was significantly positively related to the quality of life and negatively associated with anxiety and depression.Sweet basil (Ocimum basilicum, 2n=4x=48) is susceptible to downy mildew caused by Peronospora belbahrii. Pb1 gene exhibit complete resistance to the disease. AZD9291 in vitro However, Pb1 became prone to disease due to occurrence of a new virulent races. Here we show that Zambian accession PI 500950 (O. americanum var pilosum) is highly resistant to the new races. From an interspecies backcross between PI 500950 and the susceptible cv ‘Sweet Basil’ we obtained, by embryo rescue, a population of 131 BC1F1 plants. This population segregated 73 Resistant 58 Susceptible (1 1, P=0.22), suggesting the resistance is controlled by one incompletely dominant gene called Pb2. To determine whether allelic relationship is existing between Pb1 and Pb2, we used two differential races, race-0, avirulent to both PI 500945 (Pb1) and PI 500950 (Pb2) and race-1, virulent to PI 500945 but avirulent to PI 500950. F1 plants obtained from ’12-4-6′ (BC6F3 derived from PI 500945) and ’56’ (BC3F3 derived from PI 500950) showed resistant superiority to both races due to dominant complementary interaction. F2 plants segregated to race-0 as follow; 1231, immune incomplete resistant susceptible, as against to 934 to race-1, indicating Pb1 and Pb2 are not alleles. Since joint action is contributed in F1 plants and in advanced (BC3F3(56) x BC6F3(12-4-6) F4) populations who carrying both genes, it can be assumed that both accessions carry two unlinked genes but share a common signal transduction pathway which leading to dominant complementation superiority of the resistance against different races of BDM.Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating citrus diseases worldwide. Generating disease-resistant citrus varieties is considered one of the most efficient and environmentally friendly measures for controlling canker. Xcc causes canker symptoms by inducing the expression of canker susceptibility gene LOB1 via PthA4, a transcription activator-like (TAL) effector, by binding to the effector binding element (EBE) in the promoter region. In previous studies, canker-resistant plants were generated by mutating the coding region or the EBE of LOB1. However, homozygous or biallelic canker-resistant plants have not been generated for commercial citrus varieties, such as grapefruit (C. paradisi), which usually contain two alleles of LOB1 and thus have two types of LOB1 promoter sequences TI LOBP and TII LOBP. Two different sgRNAs were used to target both EBE types. Both 35S promoter and Yao promoter were used to drive the expression of SpCas9p to modify EBEPthA4-LOBP in grapefruit.