Activiteit

Mast cells play an important role in allergic responses and persistently exposure to environmental fine particulate matter (PM2.5) exacerbates allergic diseases,but the details remained elucidative.

To investigate the effect of PM2.5 on IgE-mediated mast cell responses through an IgE-mediated mouse model and mast cell activation.

The β-hexosaminidase release and a BALB/c model of passive cutaneous anaphylaxis (PCA) was used to test IgE-mediated mast cells activation in vitro and in vivo. RNA-Seq technique was conducted to study the gene expression profile. Reactive oxygen species (ROS) production was measured by flow-cytometry. RT-PCR,WB and ELISA were performed to examine targeting molecules expression.

PM2.5 facilitated IgE-mediated degranulation and increased cytokines expression in mast cells. Meanwhile, the Evan’s blue extravasation as well as serum cytokines in mice was increased after treatment with PM2.5. Furthermore, PM2.5 treatment dramatically increased the expression of Gadd45b which is an oxidative stress molecule that directly activates down-stream pathway, such as MEKK4/JNK. PM2.5 treatment activated MEKK4, JNK1/2 but not ERK1/2 and p38. Meanwhile, Knockdown of Gadd45b significantly attenuated PM2.5-mediated JNK1/2 activation and expression of cytokines. Zongertinib HER2 inhibitor In addition, a JNK1/2-specific inhibitor SP600125 blocked IgE-mediated mast cell activation and cytokine release in PCA model mice. Moreover, PM2.5 treatment increased the ROS level and ROS inhibitor dramatically blocked the PM2.5-induced ROS production and reversed the PM2.5-mediated gene expression in the mitochondrial respiratory chain.

PM2.5 regulates ROS production through Gadd45b/MEKK4/JNK pathway, facilitating IgE-mediated mast cell activation.

PM2.5 regulates ROS production through Gadd45b/MEKK4/JNK pathway, facilitating IgE-mediated mast cell activation.

The involvement of the nerve in psoriasis development was suggested by sporadic case reports.

To provide multiple evidence for the nerve in psoriasis development with a retrospective case review, a literature review and a mouse-based experimental experiment.

Psoriatic patients who had concomitant nerve injuries and such cases from literatures were reviewed. And, on wild-type mouse level, unilateral denervation surgery was performed on the dorsal skin before and after the induction of psoriasiform dermatitis, respectively. Lesion visual scores were calculated, and biopsies were taken for hematoxylin-eosin (HE) staining, immunofluorescence analysis, and RNA sequencing & bioinformatics analysis before denervation surgery and the 2

, 4

, 6

, 8

day after the surgery.

All clinical cases (20/20) showed that local lesions under the control of injured nerves relieved spontaneously or even cleared/spared, and only about 1/3 experienced partial recurrence. Next, mouse psoriasiform experiments demonstratignaling and Th17 cell differentiation, may be the mechanisms of the nerve in psoriasis.

The CAT-T was incorporated into an interprofessional error disclosure (ED) simulation to assess team communication with a patient family member.

Interprofessional student groups (n = 55) planned for team-based ED with facilitation by faculty. Students participated in three scenarios of ED with a standardized family member (SFM) who displayed the emotions of sadness, anger, and distrust sequentially. SFMs completed the CAT-T tool for each emotion. CAT-T items were classified as empathy or disclosure skills by factor analysis. Qualitative comments from the SFM were provided during debrief.

Students performed well on team-based ED from the SFM perspective. Team performance improved when the SFM displayed distrust after observing peers disclose for the sad and angry emotion. Higher composite scores were found on CAT-T items categorized as empathy skills.

The ED simulation allowed students to practice disclosure skills in a low-stakes environment and receive feedback from a SFM based on behaviors evaluated on the CAT-T. The simulation design allowed students to improve their performance by incorporating feedback into subsequent cycles.

Immersive experiences where observable student behaviors can be assessed are important to enhance IPE curriculum and to build skills in pre-licensure students that will be foundational for collaborative practice.

Immersive experiences where observable student behaviors can be assessed are important to enhance IPE curriculum and to build skills in pre-licensure students that will be foundational for collaborative practice.

Primary liver cancer is a public health problem in Mexico and the world. Liver transplantation (LT) is the ideal treatment for early hepatocellular carcinoma (HCC). Our aim was to evaluate the characteristics of patients with HCC and cholangiocarcinoma (CC) at two centers and identify transplantation candidates.

A retrospective observational study was conducted at the Hepatology Center (HC) and the University Center Against Cancer (UCAC), within the time frame of 2012-2018. HCC or intrahepatic CC was confirmed in 109 patients. Staging classifications, transplant selection models, and a predictive model for post-LT recurrence were applied to the HCC patients.

Of the total population, 93% (n = 102) presented with cirrhosis, 86% (n = 94) had HCC (HC 58%, UCAC 42%), and 14% (n = 15) had intrahepatic CC (HC 40%, UCAC 60%). Of the HC patients with HCC, Okuda I-II, BCLC A-B, and AFP levels < 100 ng/m predominated, whereas Okuda II-III, BCLC C-D, and AFP levels > 1,000 ng/mL predominated in the UCAC patients. Half of the HC population with HCC met the criteria for LT, in contrast to 23% of the UCAC patients. Fifteen patients were evaluated for LT, and at present, six have undergone transplantation.

The most frequent primary liver tumor was HCC. Patients from the HC presented with earlier-stage disease and a high number of them met the criteria for LT. Only patients from the HC underwent transplantation.

The most frequent primary liver tumor was HCC. Patients from the HC presented with earlier-stage disease and a high number of them met the criteria for LT. Only patients from the HC underwent transplantation.